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Gross and histopathological evaluation of the hepatoprotective and nephroprotective properties of native guava (Psidium guajava Linn. var. native guava) leaf extract on CCL4-fed white mice (Mus musculus).


R-Jay Hombrobueno,
Vernadyn Morillo

Related Institution

Department of Veterinary Medicine - Nueva Vizcaya State University

Publication Information

Publication Type
Publication Sub Type
Journal Article, Original
NVSU Research Journal
Publication Date
January-June 2014
Vol. 1
No. 1


The Native Guava (Psidium guajava Linn. var. native guava) Leaf Extract was evaluated for its hepatoprotective and nephroprotective properties induction of acute liver and kidney damage in laboratory mice using carbon tetrachloride (CCl4). The mice were orally administered with 1ml/200g body weight 3% CCl4 mixed in corn oil and after 24 hours, the mice were sacrificed and the liver and kidney were harvested for gross and histopathologic evaluation.

It was found that oral supplementation of native guava leaf extract (NGLE) at a concentrartion of 200mg/kg body weight prevented enlargement of both liver and kidney. Evaluation of macroscopic or gross lesions in both organs showed marginalor minimal presence of lesions. Histopathological evaluation of liver and kidney showed fewer numbers of observed cellular lesions with lesser degree and extent of tissue damage when compared with animals that did not receive any NGLE supplementation. This study was able to show that NGLE has hepatoprotective and nephroprotective properties and can be used to prevent kidney and liver damage.


This study aimed to determine the efficacy of native guava leaf extract as hepatoprotective and nephroprotective agent. Specifically, it 1) determined the effect of administration of NGLE at a concentration of 200mg/kg BW on liver and kidney organ weights; 2) assessed the number and extent of gross/macroscopic kidney and liver lesions; and 3) evaluated the severity and extent of histopathologic/microscopic kidney and liver lesions of mice after induction of acute kidney and liver damage using carbon tetrachloride (CCl4).


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2. Chanchal, R. K., Kamath, J. V., Assad, M.. "Hepatoprotective activity of Psidium guajava Linn. Leaf Extract." Indian Journal of Experimental Biology Vol. 44(No. 4): 305-311, . Retrieved from: http://nopr.niscair.res.in/handle/123456789/6438
3. Morton , Julia . Guava. Fruits of Warm Climates Miami, Florida: Julia F. Morton, 1987. 356-363. . Retrieved from: http://www.hort.purdue.edu/newcrop/morton/guava.html

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